Schematic of the experimental sSAXS setup and data processing steps. (a) The mouse head is irradiated at select locations with a collimated beam of polychromatic X rays of 1 mm diameter. A 2D spectroscopic detector (CdTe) is used to collect SAXS data simultaneously in angle- and energy-dispersive modes for each location. The sample to detector distance (SDD) was 214 mm (b) sSAXS data processing steps in 30–45 keV energy range are illustrated using an amyloid model from BSA with two Bragg peaks at 6.04 and 13.24nm−113.24nm−1. The 2D detector data (left) showing counts in each pixel per energy bin is converted to scattering cross section, S(q), per energy bin (middle) after applying a transmission correction. The S(q) is then summed (Δq=1.2nm−1Δq=1.2nm−1) from 30 to 45 keV to combine scattering information in all energy bins and to calculate the area under the peak (AUP) from 3.6 to 8.4nm−18.4nm−1 (right). Credit: https://www.nature.com/articles/s41598-020-77554-5/figures/1

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Researchers aim to advance understanding of Alzheimer's disease progression

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